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Dr. rer. nat. Ulrich Weininger

Publications PhD thesis   Structural charcterisation of protein folding   intermediates with NMR-spectroscopy E-mail:

Interests

• Dynamic of aromatic side chains in proteins

• Dynamics of catalytic residues
• Hierachy  of the processes in the active sites of  enzymes

• Protonation kinetics in tyrosines and histidines
• Transient tautomerisation of histidines -  influence  on the hydrogen bond network

• Ring-flips as probes of protein breathing
• Ring-flips as probes of aromatic interaction strength

• pK_a values in proteins
• Dynamics of methyl- and carboxylgroups in proteins
• Protein folding and misfolding
• Protein folding intermediates
• Structure determination of proteins in solution

Methods

• selective ¹³C labeling of proteins
• relaxation optimized NMR spectroscopy
• protein dynamics by NMR-spectroscopy:
  • R₂ relaxation dispersion (up to 2 000 s^-1)
  • R_1ρ relaxation dispersion (up to 30 000 s^-1)
  • R₂ vs pH experiments (up to 2 000 000 s^-1)

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