Alexey Krushelnitsky
Address:University of Halle, Institute of Physics, NMR group,
Betty-Heimann-Str. 7, 06120, Halle (Saale), Germany
Tel: +49 (345) 5528564
e-mail: Krushelnitsky@physik.uni-halle.de
Birth: 1964, Kazan, Russia
Education
1986: Diploma with excellence in radiophysics, Physics Department, Kazan
State University.
1991: Candidate of Physical and Mathematical Sciences (PhD),
Kazan State University. The Thesis: "Nuclear magnetic
relaxation and molecular motions in solutions of light globular proteins".
2007: Doctor of Physical and Mathematical Sciences (Habilitation), Kazan State University. The thesis: "Molecular dynamics of proteins and polypeptides. The
relaxation and exchange NMR study".
Professional experience
1986 - 2011: Postgraduate Student - ... - Leading
Researcher, Kazan Institute of Biochemistry and Biophysics of Russian Academy
of Sciences, Kazan, Russia.
2010 - 2011: Docent (part time employment), Kazan State University, Microbiology Department, Kazan, Russia.
2011 - present: Postdoctoral fellow, Staff scientist, Martin-Luther University of Halle-Wittenberg,
Faculty of Natural Sciences II, Institute of Physics, Halle, Germany.
Research interests
Protein molecular dynamics in the solid and liquid states,
application of various NMR techniques to biopolymer studies
Temporary work outside home laboratory
- 10/1991 – 12/1991: Ulm University, Section NMR, Ulm, Germany. Host: Prof. Rainer Kimmich
- 10/1992 – 9/1993: Stanford University, Stanford Magnetic Resonance Laboratory, Stanford, USA. Host: Prof. Oleg Jardetzky
- 4/1999 – 8/1999: University of Minnesota, Minneapolis, USA. Host: Prof. Kevin Mayo
- 1996 – 2011: two to five months long visits each year to University of Halle, Physics Department, NMR group, Halle, Germany. Hosts: Prof. Horst Schneider, Prof. Detlef Reichert, Prof. Kay Saalwaechter
- 2/2008, 9/2009: Leibniz Institute of Molecular Pharmacology, Berlin-Buch, Germany. Host: Prof. Bernd Reif
Most notable achievements
- Elaboration of the proton-decoupled R1rho experiment under MAS
- Detection of the "long tail" of the protein Brownian tumbling correlation function arising due to the inter-molecular electrostatic steering in solution
- Revealing the importance of anisotropic inter-protein interactions for the (de)coupling of rotational and translational diffusions in concentrated protein solutions
- The first application of the solid-state NMR MAS-synchronized exchange experiments to the study of the millisecond time-scale dynamics of proteins
-
Reichert D., Krushelnitsky A.
CODEX-based methods for studying slow dynamics.
in: Modern methods in solid-state NMR: a practitioner's guide, P. Hodgkinson Ed., Royal Society of Chemistry, 2018 (ISBN 978-1-78262-854-5), pp. 161-192. -
Linser R., Sarkar R., Krushelnitzky A., Mainz A., Reif B.
Dynamics in the solid-state: perspectives for the investigation of amyloid aggregates, membrane proteins and soluble protein complexes.
Journal of Biomolecular NMR, 2014, 59, 1-14. -
Krushelnitsky A., Reichert D., Saalwaechter K.
Solid-state NMR approaches to internal dynamics of proteins: from picoseconds to microseconds and seconds.
Accounts of Chemical Research, 2013, 46, 2028-2036. - Krushelnitsky A.
Complex NMR approaches to studying conformational dynamics of biopolymers.
in Biopolymer Research Trends, T.S. Nemeth Ed., Nova Publishers, New York, 2008 (ISBN 1-60021-983-7), pp. 87-118. - Krushelnitsky A.
Intermolecular electrostatic interactions and Brownian tumbling in protein solutions.
Physical Chemistry Chemical Physics, 2006, 8, 2117-2128. - Krushelnitsky A., Reichert D.
Solid state NMR and protein dynamics.
Progress in Nuclear Magnetic Resonance Spectroscopy, 2005, 47, 1-25. - Krushelnitsky A.
Exchange solid state NMR spectroscopy: the application for the large-scale conformational biopolymer dynamics study.
Uspekhi Fizicheskikh Nauk (Moscow), 2005, 175, 815-832.
English translation: Physics - Uspekhi, 2005, 48, 781-796.
-
Krushelnitsky A., Hempel G., Jurack H., Ferreira Mendes T.
Rocking motion in solid proteins studied by the 15N proton-decoupled R1rho relaxometry.
Physical Chemistry Chemical Physics, 2023, 25, 15885-15896. -
Krushelnitsky A., Hempel G., Golitsyn Y.,
Wurl A., Saalwaechter K., Reichert D.
Trajectory-Based Approach for the Analysis of CODEX Solid-State Exchange Experiments in the Slow and Intermediate Motion Regime: Comparison of Experiment, Simulation, and Analytical Treatment.
Journal of Physical Chemistry C, 2021, 125, 6839-6850. -
Krushelnitsky A., Saalwaechter K.,
Relaxation-induced dipolar exchange with recoupling (RIDER) distortions in CODEX experiments
Magnetic Resonance, 2020, 1, 247-259. - Camilles M., Link S., Balbach J., Saalwaechter K., Krushelnitsky A.
Quantitative NMR study of heat-induced aggregation of eye-lens crystallin proteins under crowding conditions.
Biochimica et Biophysica Acta - Proteins and Proteomics, 2018, 1866, 1055-1061.
Corrigendum. Biochimica et Biophysica Acta - Proteins and Proteomics, 2019, 1867, 453-454. -
Krushelnitsky A., Gauto D., Rodriguez Camargo, D.C., Schanda P.,Saalwaechter K.,
Microsecond motions probed by near-rotary-resonance R1rho 15N MAS NMR experiments: the model case of protein overall-rocking in crystals
Journal of Biomolecular NMR, 2018, 71, 53-67. -
Roos M., Ott M., Hofmann M., Link S., Rossler E., Balbach J., Krushelnitsky A., Saalwaechter K.,
Coupling and decoupling of rotational and translational diffusion of proteins under crowding conditions
Journal of the American Chemical Society, 2016, 138, 10365-10372. -
Roos M., Hofmann M., Link S., Ott M., Balbach J., Rossler E., Saalwaechter K., Krushelnitsky A.,
The “long tail” of the protein tumbling correlation function: observation by 1H NMR relaxometry in a wide frequency and concentration range
Journal of Biomolecular NMR, 2015, 63, 403-415. -
Roos M., Link S., Balbach J., Krushelnitsky A., Saalwaechter K.,
NMR-detected Brownian dynamics of aB-crystallin over a wide range of concentrations
Biophysical Journal, 2015, 108, 98-106. -
Krushelnitsky A., Zinkevich T., Reif B., Saalwaechter K.,
Slow motions in microcrystalline proteins as observed by MAS-dependent 15N rotating-frame NMR relaxation.
Journal of Magnetic Resonance, 2014, 248, 8-12. -
Zinkevich T., Chevelkov V., Reif B., Saalwaechter K.,Krushelnitsky A.
Internal protein dynamics on ps to us timescales as studied by multi-frequency 15N solid-state NMR relaxation.
Journal of Biomolecular NMR, 2013, 57, 219-235. - Reichert D.,
Zinkevich T., Saalwaechter K.,Krushelnitsky A.
The relation of the X-ray B-factor to protein dynamics: insights from recent dynamic solid-state NMR data.
Journal of Biomolecular Structure and Dynamics, 2012, 30, 617-627. - Hackel C.,
Zinkevich T., Belton P., Achilles A., Reichert D., Krushelnitsky A.
The trehalose coating effect on the internal protein dynamics.
Physical Chemistry Chemical Physics, 2012, 14, 2727-2734. - Kurbanov R., Zinkevich T.,Krushelnitsky A.
The nuclear magnetic resonance relaxation data analysis in solids: General R1/R1rho equations and the model-free approach.
Journal of Chemical Physics, 2011, 135, 184104. - Krushelnitsky A., Zinkevich T., Reichert D.,
Chevelkov V., Reif B.
Microsecond time scale mobility in a solid protein as studied by the 15N R1rho site-specific NMR relaxation rates.
Journal of the American Chemical Society, 2010, 132, 11850-11853. - Krushelnitsky A., deAzevedo E., Linser R., Reif B.,
Saalwaechter K., Reichert D.
Direct observation of millisecond to second motions in proteins by dipolar CODEX NMR spectroscopy.
Journal of the American Chemical Society, 2009, 131, 12097-12099. - Krushelnitsky A., Zinkevich T., Mukhametshina N., Tarasova
N., Gogolev Y., Gnezdilov O., Fedotov V., Belton P., Reichert D.
13C and 15N NMR study of the hydration response of T4 lysozyme and aB-crystallin internal dynamics.
Journal of Physical Chemistry B, 2009, 113, 10022-10034. - Krushelnitsky A., Mukhametshina N., Gogolev Y., Tarasova
N., Faizullin D., Zinkevich T., Gnezdilov O., Fedotov V.
Subunit mobility and the chaperone activity of recombinant aB-crystallin.
The Open Biochemistry Journal, 2008, 2, 116-120. - Krushelnitsky A., Gogolev Y., Golbik R., Dahlquist F.,
Reichert D.
Comparison of the internal dynamics of globular proteins in the microcrystalline and rehydrated lyophilized states.
Biochimica et Biophysica Acta - Proteins and Proteomics, 2006, 1764, 1639-1645. - Krushelnitsky A., Brauniger T., Reichert D.
15N spin diffusion rate in solid-state NMR of totally enriched proteins: the magic angle spinning frequency effect.
Journal of Magnetic Resonance, 2006, 182, 339-342. - Krushelnitsky A., Reichert D.
Complex 1H, 13C NMR relaxation and computer simulation study of side chain dynamics in solid polylysine.
Biopolymers, 2005, 78, 129-139. - Krushelnitsky A., Reichert D.
Response of lysozyme internal dynamics to hydration probed by 13C and 1H solid-state NMR relaxation.
Applied Magnetic Resonance, 2004, 27, 501-518. - Krushelnitsky A., Faizullin D., Reichert D.
Hydration dependence of backbone and side chain polylysine dynamics: A 13C solid-state NMR and IR spectroscopy study.
Biopolymers, 2004, 73, 1-15. - Krushelnitsky A., Hempel G., Reichert D.
Simultaneous processing of solid-state NMR relaxation and 1D-MAS exchange data: the backbone dynamics of free vs. binase-bound barstar.
Biochimica et Biophysica Acta - Proteins & Proteomics, 2003, 1650, 117-127. - Krushelnitsky A., Kurbanov R., Reichert D., Hempel G., Schneider H.,
Fedotov V.
Expanding the frequency range of the solid state T1rho experiment for heteronuclear dipolar relaxation.
Solid State NMR, 2002, 22, 423-438. - Ermakova E.A., Krushelnitsky
A.G., Fedotov V.D.
Brownian dynamics simulation of electrostatically interacting particles.
Molecular Physics, 2002, 100, 2849-2855. - Krushelnitsky A., Mefed A., Kharitonov A, Fedotov V.
Study of slow molecular motions in alpha-crystallin by proton magnetic spin-lattice relaxation in the doubly rotating frame.
Applied Magnetic Resonance, 2001, 20, 207-229. - Nesmelova I., Krushelnitsky A.,
Idiyatullin D., Blanco F., Ramirez-Alvarado M., Daragan V., Serrano L.,
Mayo K.
Conformational exchange on the microsecond time scale in a-helix and b-hairpin peptides measured by 13C NMR transverse relaxation.
Biochemistry, 2001, 40, 2844-2853. - Idiyatullin D., Krushelnitsky A.,
Nesmelova I., Blanco F., Daragan
V., Serrano L., Mayo K.
Internal motional amplitudes and correlated bond rotations in an a-helical peptide derived from 13C and 15N NMR relaxation.
Protein Science, 2000, 9, 2118-2127. - Krushelnitsky A.G., Reichert D., Hempel G., Fedotov V.D., Schneider H.,
Yagodina L.O, Schulga A.A.
Superslow backbone protein dynamics as studied by 1D solid-state MAS exchange NMR spectroscopy.
Journal of Magnetic Resonance, 1999, 138, 244-255. - Krushelnitskii
A.G., Yudina E.V., Fedotov
V.D.
The proton relaxation study of the Brownian dynamics of Bovine Serum Albumin.
Molekulyarnaya Biologya (Moscow), 1997, 31, 293-299. - Krushelnitsky A.G., Fedotov V.D., Spevacek J., Straka J.
Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study.
Journal of Biomolecular Structure and Dynamics, 1996, 14, 211-224. - Krushelnitskii
A.G.
The study of trp-repressor dynamics in solution by means of non-selective proton relaxation.
Molekulyarnaya Biologiya (Moscow), 1996, 30, 631-636. - Faizullin D.A., Stupishina E.A., Krushelnitskii A.G., Fedotov V.D.
Examination of dynamic properties of Binase in solution by hydrogen exchange and 1H NMR relaxation.
Molekulyarnaya Biologiya (Moscow), 1995, 29, 563-573. - Ermolina I.V., Krushelnitsky
A.G., Ivoylov I.N., Feldman Yu.D., Fedotov V.D.
Investigation of molecular motions and interprotein interactions by NMR and TDDS.
Applied Magnetic Resonance, 1993, 5, 265-283. - Krushelnitsky A.G., Fedotov V.D.
Overall and internal protein dynamics in solution studied by the nonselective proton relaxation.
Journal of Biomolecular Structure and Dynamics, 1993, 11, 121-141. - Krushelnitskii
A.G., Fedotov V.D.
Effect of electrostatic intermolecular interactions on Brownian rotation of proteins in solution.
Moleculyarnaya Biologiya (Moscow), 1992, 26, 424-431. - Fedotov V.D., Feldman Yu.D., Krushelnitsky A.G., Ermolina I.V.
NMR and dielectric spectroscopy investigation of protein dynamical structure.
Journal of Molecular Structure, 1990, 219, 293-298. - Kivayeva L.S., Krushelnitsky
A.G., Fedotov V.D.
On mechanisms of water nuclear magnetic relaxation in protein solutions. .
Physiologie, 1989, 26, 297-303.